Gastric cancer xenograft mouse models

Ion4,5, Carmen C. Diaconu1 1 Stefan S. Hofigal Export Import S.

Referințe bibliografice pe an

Cytotoxicity is the first that should be ruled out when searching for an antiviral entity. Therefore, first we investigated the potential cytotoxicity of these compounds, in order gastric cancer xenograft mouse models eliminate this gastric cancer xenograft mouse models in the analysis of the virucidal effect. Methods: We have used different methodologies for testing cytotoxicity microscopy, flowcytometry, 3D cell culture and a set of European standardized procedures for the virucidal activity testing in human medicine.

The standard impose strict testing methods on two non-enveloped viruses, one DNA and one RNA, highly resistant and stable in different conditions Adenovirus type 5, Poliovirus type 1.

Hericium erinaceus HE has been used for the treatment of digestive diseases for over years in China. HE possesses many beneficial functions such as anticancer, antiulcer, antiinflammation and antimicrobial effects, immunomodulation and other activities. The aim of the studies was to evaluate the anticancer efficacy of two extracts HTJ5 and HTJ5A from the culture broth of HE against three gastrointestinal cancers such as liver, colorectal and gastric cancers in both of in vitro of cancer cell lines and in vivo of tumor xenografts and discover the active compounds. Tumor volumes and body weight were measured daily during the first 10 days and times a week thereafter to assess the tumor growth inhibition, tumor doubling time, partial and complete tumor response and toxicity.

Results: All tested natural bioactive compounds T gallica, S. One compound T. T gallica extract reduced the poliovirus infectivity to a lgTCID50 of 5. The other natural bioactive extracts tested showed less cytotoxicity compared to T. However, the combination of T. Conclusion: Virucidal activity demonstrated especially by T.

Acknowledgements: Ioana M. The significant increase in the number of thyroid carcinoma-diagnosed patients and its aggres- siveness prompted us to utilize high resolution mass spectrometry methodology to identify the patient's tumor proteomic profile that could facilitate the understanding gastric cancer xenograft mouse models tumor biogenesis and help in prognosis and therapy.

The study aimed at the identification of molecular markers of the follicular adenoma or papillary thyroid carcinoma by differential proteomic analysis. Materials and methods. Thyroid tissues sampled from the operation theatre gastric cancer xenograft mouse models collected from two groups of female patients: 1.

Control CD and CP respectively tissue samples adjacent to the tumors were also collected and analyzed from each specimen. The tissue homogenates were processed for liquid nano-chromatography mass spectrometric analysis.

Supliment II JTMR

The statistically significant differentially expressed proteins matched with KEGG databases revealed the over-representation of the proteins involved in thyroid hormone synthesis inter-relation map. Bioinformatics gene ontology gastric cancer xenograft mouse models Protein Center 3. In addition, they warts treatment in homeopathy involved in cellular response to stress and binding chaperone molecules.

Altogen Labs NCI-N87 Xenograft Service Gastric Cancer

The relative quantification of these proteins by label free mass spectrometry was validated by immunological assays and correlated with their serum expression levels. The papilloma removal mouth spectrometry analysis revealed significant differences in protein expression in follicular adenoma versus papillary thyroid carcinoma that could help in early prognosis and adequate treatment decision.

Cercetarea moleculară are un rol important în descifrarea gastric cancer xenograft mouse models unor boliîn introducerea unor noi metode de investigaţii și tratament si în identificarea subiecţilor cu risc crescut. Mă voi opri numai la 2 domenii importante și anume genetica si imagistica moleculara.

Genetica moleculară a intrat progre- siv și definitiv în multe domenii ale medicinii. In cardiologie întâlnim cel mai frecvent afecţiuni monogenice și putem vorbi de 3 mari clase de modificări genetice ale proteinelor: modificări structurale, modificări electrice și modificări de reglare.

Modificări structurale întâlnite în cardiomiopatii, tezaurismoze, sindromul Marfan etc; modificări ale proteinelor ţesutului electric: sindromul QT lung. Să luăm un exemplu. Cardiomiopatia hipertrofică este produsă de modificări ale genelor proteinelor sarcometrie, din care 3 sunt mai S4 Journal of Translational Medicine and Research 20 Supplement IIimportante cu o heterogenitate intra genică f.

Determinarea genetică este recomandată la probanţi, pentru a afla ce mutaţie este prezentăşi la rudele de gr. I pentru a afla care dintre acestia gastric cancer xenograft mouse models hpv uomo test boala. La început, în urmă cu ani,entuziasmul era foarte mare, vom afla cine produce boala și mai ales care din descendenţi va face boala. Ulterior am constatat că nu putem preveni bola şi nici nu putem lua decizii terapeutice dacăștim modificarea genetică.

Cu totul altă situaţie gastric cancer xenograft mouse models cu Boala Fabri unde s-a descoperit enzima ce blochează depozitarea de ceramid în lizozomii celulelor mio- cardice, renale, piele. Astfel impactul clinic al cercetării genetice în cardiologie este mult sub speranţele noastre privind prevenirea şi tratamentul unor boli.

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Exclud impactul asupra cunoasterii. Imagistica moleculară se adaugă imagisticii structurale. Aduce date noi din gastric cancer xenograft mouse models celulelor vii, in timp real, invizibile cu alte metode; implicate in diverse procese patologice. Daca mă refer numai la ateroscleroza: avem multe neinpliniri privnd prevenirea apariţiei plăcii de aterom, nu ştim prea bine cum,dece şi mai ales când se fisurează placa de aterom.

Stăm destul de bine la tratamentul plăcii fisurate infarctul miocardic si prevenirea unui nou episode acut.

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Ne intrebăm dacă imaginile moleculare pot modifica atitudinea medicului şi a bolnavului. Datele publicate până în prezent sunt încurajatore privind rolul efortului fizic,al statinelor si IECA. Ştim bine astazi rolul inflamatiei în afecţiunile cardiovasculare dar nu avem încă un tratament eficient al inflamaţiei,iar gastric cancer xenograft mouse models sunt mici. Cercetarea medicală necesită costuri imense. In SUA se cheltuiesc 30 miliarde anual pentru cercetarea medicala. Ne punem uneori intrebarea daca cercetarea medicală se face pentru ingrijirea mai buna a bolnavilor sau pentru business?

Am aici în vedere tratamentul hipertensiunii arteriale,dar nu numai. Tehnologia actualatotusi departe de scanerul Dr.

Volume Articles

Early detec- tion of pancreatic cancer could decreases the mortality caused by this disease. The main focus is deciphering the molecular biology of this tumor type, in order to identify new factors related to pancreatic oncogenesis.

However, nuclear β-catenin protein accumulation has been correlated with late stages of tumor progression and metastasis A major role in genes regulation is epigenetic modification, especially DNA methylation.

Identification of genes which undergo cancer-specific methylation changes and correlation of these data with tumor stage, progression, and long-term prognosis are becoming increasingly common.

Another key factor for β-catenin gene regulation is ERBB2 which is over expressed in several cancer types.

The aim of this study was to identify the factors involved in β-catenin gene expression deregulation in pancreas oncogenesis. We found a decreasing methylation pattern of β-catenin promoter gene related to the disease stage. Fold change expression for β-catenin was between 0. Gastric cancer xenograft mouse models results sustain the increased β-catenin gene expression trough epigenetic alterations demethylation of gene promoter and the increasing of ERBB2 gene expression, β-catenin regulatory factor.

Therefore, these factors may represent a trigger for pancreatic oncogenesis process. Urmare a acestui proces, celulele sistemului imunitar de exemplu, monocitele sunt atrase şi participa la transmigrarea celulelor tumorale printre CE si la formarea metastazelor.

Carmen Diaconu

Datorita implicarii chemokinelor în metastazarea cancerelor de diferite origini, intervenţia gastric cancer xenograft mouse models asupra sistemului de chemokine deschide noi oportunitati în tratamentul unor tipuri de cancer şi prevenirea metastazarii. Scopul acestui studiu a fost de a testa daca directionarea liposomilor tinta-senzitivi TSL care transporta inhibitori ai receptorilor chemokinici si ii elibereaza gastric cancer xenograft mouse models la situsuri cu endoteliu activat reduc metastazarea celulelor tumorale.

Rezultatele au aratat ca in vitro, Vp-TSL-Tj se leagă specific de suprafata CE gastric cancer xenograft mouse models unde elibereaza compusul incorporat; acesta determina reducerea transmigrarii monocitelor si a celulelor tumorale prin endoteliul activat.

Interesant si incurajator, doua administrari de liposomi Vp-TSL-Tj in soareci cu o ora inainte si la 16 ore dupa injectarea celulor tumorale a determinat o scadere semnificativa a metastazelor in plaman la 28 de zile de la injectarea celulelor tumorale.

Aceste rezultate sunt o prima dovada ca nanocarausii care transporta și eliberează inhibitori de chemokine CCR2 la situsurile vasculare cu endoteliu activat reduc metastaza celulelor tumorale. The disease is preventable by a live attenuated vaccine, that provides lifelong immunity in most juvenile papillomatosis laryngeal. A national measles vaccination program was implemented in Romania in an initiative of academician Nicolae Cajal.

Vaccination was conducted with an indigenous S6 Journal of Translational Medicine and Research 20 Supplement IImonovalent measles-containing vaccine Schwarz strain produced by Cantacuzino Institute. Nevertheless, during the last decade,Romania experienced at least two important measles outbreaks.

Articles of Volume : 54 Issue : 1, March, Dynamics of histological changes in traumatized liver tissues in the absence of alcohol intoxication Author : Olena P. Liver injury is an important cause of death in patients with traumatic injuries.

The first one started in December and lasted until early More than 9, cases were detected, frequently in non-immunised patients belonging to the Roma ethnic group. The second major epidemic episode occurred duringsimultaneously with a rubella epidemic in In contrast to other vaccine - preventable diseases, most cases of measles reflect a failure to receive gastric cancer xenograft mouse models. Failure to introduce appropriate infection-control measures,as well as the potential for and transborder viral transmission are alternative explanations for the extenssion of the transmission chain.

Enteroendocrine cells in the gut produce hormones that play essential roles in the regulation of food intake, energy expenditure and glucose homeostasis. Analogues of the gut hormone glucagon-like peptide-1 GLP-1 are widely used for the treatment of T2DM, and are under development for the treatment of obesity.

Recent findings, showing that many cases of T2DM resolve after gastric bypass surgery, have triggered a global interest in the gut endocrine system, and whether it could be harnessed for the treatment of diabetes and obesity.

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A body of evidence supports the idea that gut microbiota influence peripheral metabolism, and it is gastric cancer xenograft mouse models that the underlying pathway includes modulation of the enteroendocrine cells with which they come into contact.

The types of enteroendocrine cells vary along the length of the intestinal tract. The colon contains the highest density of enteroendocrine L cells together with most of the gut bacteria. Here, we reveal that indole, a metabolite produced from the dissimilation of tryptophan, is able to modulate the secretion of GLP-1 from immortalized and primary mouse colonic Lcells and we reveal the molecular mechanism behind this modulation [1].

Indole stimulates GLP-1 secretion over short time scales and gastric cancer xenograft mouse models reduces the rate of GLP-1 secretion over longer periods. Our results identify indole as a signalling molecule by which gut microbiota communicate with Lcells and influence host metabolism. Reference: [1] C.

Chimerel, E. Emery, D. Summers, U. Keyser, F. Gribble, F.

Citate duplicat

Reimann, Bacterial metabolite indole modulates incretin secretion from intestinal enteroendocrine L cells, Cell reports, 9 Ciurea1,2, H. Journal of Translational Medicine and Research 20 Supplement IIS7 Materials and methods: Classical physics describes motion to be the change in position of an object with respect to time and its refference point. Motion in the universe is described using two apparently contradictory sets of laws.

The motion of familliar objects — including cells — are described by classic mechanics while the movement of atoms and subatomic particles are described by quantum mechanics. Biological motility is the capacity of an organism to move spontaneously and voluntary at the expense of energy and represents one of the crucial traits if not the most important trait of all living matter.

Motility is a key process present in unicellular organisms as well as multicellular organisms and is the basis of embryonic development, healing, immune response, tumor formation, metastasis, cell migration, digestion, circulation, respiration and reasoning and various other critical processes including biological evolution itself. As creatures evolved, various types of motility were developed — these include chemotaxis motility along a chemical gradientthermotaxis motility gastric cancer xenograft mouse models a temperature gradientphototaxis motility along a light gradientmagnetotaxis motility along a magnetic fieldgalvanotaxis motility along an electrical fieldgravitaxis motility along the direction of gravitational gastric cancer xenograft mouse models motility along a rigidity gradienthaptotaxis motility along a gradient of cell adhesion sites and many others.

gastric cancer xenograft mouse models

Nowadays researchers only begin to understand the crucial part motility played in the evolution of species. In all areas of surgery cell and tissue motility is singlehandedly the key to healing and succes.

The constant motility of developing organisms from trilobites to human beings has undoubtedly shaped evolution by constantly improving the functions of the skeletal system and other internal organs such as the digestive tract, liver, kidneys etc. The human central nervous system makes no exception from this rule.

The development of the human nervous system takes place by employing chemo- taxis, galvanotaxis, magnetotaxis and haptotaxis. The human heart is constantly moving blood throughout the body ensuring the correct metabolism of living tissues. Oxygenated red blood cells come from the lungs to the tissue filled with life-giving oxygen while red cells filled with carbon dyoxide are moved from the tissue to the lungs for the cycle of respiration.

Blood moves through larger veins and arteries at speeds of about 0. Smooth muscles of the body are also constantly moving. The ear is another organ functioning based on motion, besides the vibration of the eardrum generating the sense of hear the inner ear is able to gastric cancer xenograft mouse models motions of the head equal to the diameter of a single hair. The human lymphatic system is constantly moving excess fluids, lipids and immune system-related gastric cancer xenograft mouse models around the body.

Intracellular cytoplasmatic streaming is responsible for the transport of key mollecules in living cells. The bipedal posture of humans enabled prehistoric humans to hunt, go fishing and even grow crops and it allowed for a continuous development of the central nervous system which was manda- tory for survival, self defense and preservation of the species.

The basic process for thought is neural network- ing which is the motion of information through neurons. This can be gastric cancer xenograft mouse models chemically or electrically. Chemical synapses use chemical mollecules to transport information while electrical synapses use massive ionic influx curents for signalling. The evolution from prehistoric human beings to modern men went through several steps during which human motility represented once more a crucial element.

Once industrial food production appeared — with the overproduction we are familiarized with, human motility became a protection factor against obesity which is one of the most problematic medical conditions of our time.

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According to WHO data, in there were more than 1. More than million people are obese while more than 40 million children suffer of this ailment. The cure? Physical activity! Swimming, Tennis, Cycling and limiting of hypercaloric food intake are the symplest and most effective ways to get rid of obesity and improve the individual quality of life and treatment for all medical patients.

The patient should firstly be able to move spontaneously and independantly and secondly he should be able to do that as soon as possible after surgery.

Pulmonary thrombembolism is therefore avoided, intestinal transit is ensured, csf transit is ensured, complete body motions are ensured and the nervous system is stress-free.

S8 Journal of Translational Medicine and Research 20 Supplement IIProvided the fact that any lesions of the brain have a tremendous impact of the motility of the patient the authors consider that the motility of the patient has a tremendous impact on the healing of the patient. The authors perform a comprehensive literature review about the effects of industrial food production and the vicious circle that is born between consumerism, reckless alimentation, a bad lifestyle and lack of movement.

At the same time the authors present their own experience concerning the importance of motility in neurosurgical patients and how it can dramatically change the outcome of patients.

gastric cancer xenograft mouse models que es virus del papiloma humano (vph)

To conclude — we believe that motility is the key to limiting and improving worldwide morbidity while also limiting the effects of other diseases and increasing the healing rates of other pathologies while also preventing thrombosis.

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